Description
The competitive Adrenaline (Epinephrine) ELISA Kit uses the microtiter plate format. Adrenaline is bound to the solid phase of the microtiter plate. Acylated adrenaline from the sample and solid phase bound adrenaline compete for a fixed number of antiserum binding sites. When the system is in equilibrium, free Antigens and free Antigens-antiserum complexes are removed by washing. The antibody bound to the solid phase adrenaline is detected by anti-rabbit IgG / peroxidase. The substrate TMB / peroxidase reaction is monitored at 450 nm. The amount of antibody bound to the solid phase adrenaline is inversely proportional to the adrenaline concentration of the sample